What is the main purpose of using a spectrophotometer in laboratory analysis?

The main purpose of using a spectrophotometer in laboratory analysis is to measure the intensity of light absorbed by a sample. This technique relies on the principle that different substances absorb light at specific wavelengths and to varying degrees. By analyzing this absorption, a spectrophotometer allows laboratory analysts to quantify concentrations of analytes in a solution, assess purity, and determine the presence of specific compounds.

Using a spectrophotometer is essential in various fields, including environmental monitoring, biochemistry, and quality control, as it provides precise and reliable data that are fundamental for both research and industry applications. The ability to quantify how much light is absorbed by a sample enables researchers to make informed conclusions about the chemical composition and characteristics of that sample.