What condition requires the sample to be in an amber bottle?

The requirement for a sample to be collected in an amber bottle is linked to its sensitivity to light exposure. In the case of UV254 samples, the aim is to analyze the sample for ultraviolet absorbance at 254 nanometers, which is often used as an indicator of organic and inorganic matter in water.

Amber bottles are specifically designed to protect light-sensitive analytes from degradation or alteration due to exposure to UV radiation. By using an amber bottle, the integrity of the sample is maintained, ensuring that the results of the UV254 analysis reflect the true composition of the sample without interference from photodegradation.

In contrast, other sample types such as HPC (Heterotrophic Plate Count), turbidity, or VOCs (Volatile Organic Compounds) may not have the same light sensitivity concerns and can be stored in different types of containers. Therefore, understanding the properties of the analytes being measured and the effects of environmental factors like light is crucial when selecting appropriate sample containers.